Study of neuronal circuit dynamics from multiple brain or spinal cord regions

Chromatone™ is a multiscope™ for simultaneous multi-region, multi-color stimulation and neuronal imaging in freely behaving animals, accompanied by its software package.

 

    • Allows functional imaging of neuronal circuits with optogenetic manipulation in up to four brain or spinal cord areas.
    • Enables the simultaneous recording of neurons at cellular resolution.
    • Capabilities of Quartet® and more.

Included with purchase:

    • Hands-on-surgical training
    • Neuronal trace extraction support
    • Device synchronization with other behavior equipment

GFP & RFP Imaging and Channelrhodopsin & ChrimsonR Optogenetic Stimulation

  • Power level emitted for each wavelength can be independently controlled in each imaging fiber optics.

 

  • Optogenetics protocols can be set by users independently and trigger off to perform behavioral tasks.

Specifications

Illumination colors in each fiber optic

470 +/- 12 nm (GFP, Channelrhodopsin optogenetics)

550 +/- 7.5 nm (RFP)

632 +/- 14 nm (ChrimsonR optogenetics)

Illumination power range at tissue

470 nm: 0 to 6 mW 

550 nm: 0 to 1 mW

632 nm: 0 to 2 mW

Illumination mode for each wavelength Green: CW for imaging (can be pulsed, if required)
Blue: Pulse, can get triggered and executes the Optogenetics protocol set by user
Red: Pulse, can get triggered and executes the Optogenetics protocol set by user
Optical Resolution 7 µm
Focusing Range or Working Distance 250 µm in brain tissue
Number of Imaging Probes 4
Diameter of GRIN Lens to Implant Customizable between 0.5 mm to 1 mm
Available Length of Lens to Enter Brain Tissue Customizable between 2.5 mm to 8 mm 
Field of View in Each Region Approximately the same as the lens diameter
Weight of Connector and Lens < 400 mg
Approx Weight of each Fibre Bundle Carried by Animal < 1 g
Bending Radius of Fiber Bundles 3 cm
Length of Imaging Fiber Bundles  84 cm
Detection Wavelength 517 +/- 13 nm and 590 +/- 20 nm
Image Size per Frame 3.2 megapixels
Number of Frames per Second 20 in continuous mode, 140 in burst mode
Length of Camera Electric Wire 2 meters
Length of LED Wire 1.5 meter