Optogenetic modulation and functional imaging of neural circuits

Study of neuronal circuit dynamics from multiple brain or spinal cord regions

ChromatoneTM is a multiscopeTM for simultaneous multi-region, multi-color stimulation and neuronal imaging in freely behaving animals and an accompanying software package.

    • Allows functional imaging of neuronal circuits with optogenetic manipulation in up to four brain or spinal cord regions.
    • Enables the simultaneous recording of neurons at cellular resolution.
    • Capabilities of Quartet® and more.
    • Hands-on-surgical training
    • Neuronal trace extraction support
    • Device synchronization with other behavior equipment

Specifications

Specifications

Illumination colors in each fiber optic 470 +/- 12 nm (GFP, Channelrhodopsin optogenetics)

550 +/- 7.5 nm (RFP)

632 +/- 14 nm (ChrimsonR optogenetics)

Illumination power range at tissue 470 nm: 0 to 6 mW

550 nm: 0 to 1 mW

632 nm: 0 to 2 mW

Illumination mode for each wavelength Green: CW for imaging

Blue: Pulse, can get triggered and executes the Optogenetics protocol set by user

Red: Pulse, can get triggered and executes the Optogenetics protocol set by user

Optical Resolution 7 µm
Focusing Range or Working Distance 250 µm in brain tissue
Number of Imaging Probes 4
Diameter of GRIN Lens to Implant 0.5 mm

or 0.7 mm

or 1 mm

Available Length of Lens to Enter Brain Tissue Customizable between 2.5 mm to 8 mm
Field of View in Each Region Approximately the same as the lens diameter
Weight of Connector and Lens < 400 mg
Approximate Weight of each Fibre Bundle Carried by Animal < 1 g
Bending Radius of Fiber Bundles 3 cm
Length of Imaging Fiber Bundles 84 cm
Detection Wavelength 517 +/- 13 nm and

590 +/- 20 nm

Image Size per Frame 3.2 megapixels
Number of Frames per Second 20 in continuous mode, 140 in burst mode
Length of Camera Electric Wire 2 meters
Length of LED Wire 1.5 meter