The Quartet® allows researchers to easily explore neuronal circuitry with its multi-region imaging and multi-modality compatibilities

A miniature fluorescence microscope optimized for neuronal calcium imaging

  • Simultaneous imaging of multiple regions in both the brain and spinal cord in freely behaving animals
  • Implantation in more than one animal allows simultaneous imaging of brains of multiple animals for social studies
  • Image neurons during behavioural experiments that require animal swimming
  • Capability of implantation at any angle allows imaging hard to reach brain areas, such as the olfactory bulb

The imaging fiber bundle connects to the implanted GRIN lens to transmit the fluorescence signal from neurons to the CMOS sensor. Quartet uses FOUR optical imaging fiber bundles and comes with four highly flexible and lightweight lens connectors. Each bundle includes 18,000 single fibers, each of which are 7.5 micron in diameter.

  • Lightweight and flexible probes allow performance of delicate memory and behaviour experiments with very minimal stress to the animal
  • Specially designed lightweight connectors allow probe-lens connection with minimal stress to the animal
  • Design allows simultaneous imaging with EEG and fMRI
  • Long electric cables allow easy lab space management
  • Lightweight and small microscope body can be placed anywhere, even hanging in mid-air (e.g. above the maze)
  • Connector design allows for simple focus adjustment that is maintained over weeks, for the duration of the experiment

Click here to view the adjustment in action

  • Easy connection/disconnection of probes

We currently offer two types of GRIN lenses for imaging different areas of the central nervous system

  • 0.7mm in diameter and 8mm long: able to reach any area of the brain in rodents or larger animals
  • 1mm in diameter: able to reach areas in the brain which are up to 2.5mm deep

Quartet In Action

Experimental set up of a freely behaving mouse with multiple regions of the brain being simultaneously calcium imaged. Neurescence Quartet® based Olfactory Avoidance Test analyze abilities of odor detection and recognition using genetically modified mice to study olfactory processing.

 

 

Neurescence Quartet® is used in T maze experiment to investigate memory and spatial learning. Here two neuronal regions of a freely behaving mouse’s brain are being simultaneously calcium imaged.

 

The mouse swims freely with no hindrance from the light-weight water-proof probes attached to its brain for calcium imaging with the Neurescence Quartet®.

 

Mice are seen socially interacting as part of a behavior experiment. They have been implanted with GRIN lenses prior to simultaneous calcium imaging with the Neurescence Quartet®.

 

In this movie you will find that a mouse with four implanted lenses shows no sign of motor movement or behavioural deficiency:

 

Specifications

 
Number of Imaging Probes 
 
 
Diameter of GRIN Lens to Implant 
 
Customizable between 0.5 mm to 1.0 mm
 
Available Length of Lens to Enter Brain Tissue 
 
Customizable between 2.5 mm to 8 mm  
 
Field of View in Each Region 
 
Approximately the same as the lens diameter 
 
Weight of Connector and Lens 
 
< 400 mg 
 
Length of Imaging Fiber Bundles 
 
84 cm 
 
Bending Radius of Fiber Bundles 
 
3 cm 
 
Approximate Weight of each Fibre Bundle Carried by Animal 
 
< 1 g 
 
Illumination Wavelength 
 
470 +/- 15 nm 
 
Detection Wavelength 
 
517 +/- 13 nm and 590 +/- 20 nm 
 
Illumination Power at Tissue 
 
0 to 1.2 mW 
 
Optical Resolution 
 
7.5 microns 
 
Image Size per Frame 
 
3.2 megapixels 
 
Number of Frames per Second 
 
20 in continuous mode, 140 in burst mode 
 
Length of Camera Electric Wire 
 
2 meters 
 
Length of LED Wire 
 
1.5 meters