The Quartet® allows researchers to easily explore neuronal circuitry with its multi-region imaging and multi-modality compatibilities

A miniature fluorescence microscope optimized for calcium imaging with single neuron resolution

  • Simultaneous imaging of multiple regions in the brain and spinal cord in freely behaving animals at single-neuron resolution
  • Lens implantation in more than one animal allows simultaneous imaging of brains of multiple animals for social interaction studies
  • Image and record from hundreds of neurons during behavioural experiments, including swimming experiments
  • Capability of implantation at any angle allows imaging hard-to-reach brain areas, such as the olfactory bulb

The Quartet® uses four highly flexible and lightweight optical imaging fiber bundles, which transmit neuronal fluorescence signals from the implanted GRIN lens-connector to the microscope body. Every bundle includes 18,000 single fibers, each of which are 7.5 microns in diameter.

  • Lightweight and flexible probes allow for memory and behaviour experiments with minimal stress to the animal
  • Specially designed lens-connectors allow easy fiber connection and disconnection, eliminating the need for anesthesia
  • Design allows simultaneous imaging with EEG and fMRI
  • Long electric cables allow easy lab space management
  • Lightweight and small microscope body can be placed anywhere, even hanging in mid-air (e.g. above the maze)
  • Connector design allows for simple focus adjustment that is maintained over weeks, for the duration of the experiment
  • Easy connection/disconnection of probes

Custom-designed GRIN lenses to image different brain and spinal cord regions

Standard sizes include:

  • 0.7mm in diameter and 8mm long: able to reach any area of the brain in rodents or larger animals
  • 1mm in diameter: able to reach areas in the brain which are up to 2.5mm deep

Our team works with labs to design sizes best-suited for their experiments.

Quartet In Action

Experimental set up of a freely behaving mouse with multiple regions of the brain being simultaneously calcium imaged. Neurescence Quartet® is being used to analyze abilities of odor detection and recognition using genetically modified mice to study olfactory processing.

 

 

Neurescence Quartet® is used in T maze experiment to investigate memory and spatial learning. Here two neuronal regions of a freely behaving mouse’s brain are being simultaneously calcium imaged.

 

The mouse swims freely with no hindrance from the light-weight water-proof probes attached to its brain for calcium imaging with the Neurescence Quartet®.

 

Mice are seen socially interacting as part of a behavior experiment. They have been implanted with GRIN lenses prior to simultaneous calcium imaging with the Neurescence Quartet®.

 

In this movie you will find a mouse with four implanted lenses shows no sign of motor movement or behavioural deficiency. There are two lenses in the dentate gyrus and two in the entorhinal cortex.

 

Specifications

 
Number of Imaging Probes 
 
 
Diameter of GRIN Lens to Implant 
 
Customizable between 0.5 mm to 1.0 mm
 
Available Length of Lens to Enter Brain Tissue 
 
Customizable between 2.5 mm to 8 mm  
 
Field of View in Each Region 
 
Approximately the same as the lens diameter 
 
Weight of Connector and Lens 
 
< 400 mg 
 
Length of Imaging Fiber Bundles 
 
84 cm 
 
Bending Radius of Fiber Bundles 
 
3 cm 
 
Approximate Weight of each Fibre Bundle Carried by Animal 
 
< 1 g 
 
Illumination Wavelength 
 
470 +/- 15 nm 
 
Detection Wavelength 
 
517 +/- 13 nm and 590 +/- 20 nm 
 
Illumination Power at Tissue 
 
0 to 1.2 mW 
 
Optical Resolution 
 
7.5 microns 
 
Image Size per Frame 
 
3.2 megapixels 
 
Number of Frames per Second 
 
20 in continuous mode, 140 in burst mode 
 
Length of Camera Electric Wire 
 
2 meters 
 
Length of LED Wire 
 
1.5 meters